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human miltenyi  (Miltenyi Biotec)


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    Miltenyi Biotec human miltenyi
    Human Miltenyi, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 111 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd133+microbead+kit/CD133+MicroBead+Kit%2C+human+-+lyophilized/pm42810345-311-91-92
    Average 95 stars, based on 111 article reviews
    human miltenyi - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Isolation:

    Article Title: Mfn1-mediated imbalanced mitochondrial dynamics promotes ovarian cancer stemness by inducing metabolic reprogramming.
    Article Snippet: properties contributing to cancer progression, disease recurrence, and drug resistance [1, 2].. Thus, understanding the precise molecular mechanism underpinning the stemness properties of ovarian cancer stem cells (OCSCs) will be relevant in developing effective strategies to combat ovarian cancer (OC).. CSCs have distinct metabolic properties compared to non-CSC tumor cells [3, 4].

    Article Title: Glioma-Associated Mesenchymal Stromal/Stem Cells Derived Exosomal miR-191 Promotes the Proneural-to-Mesenchymal Transition in Glioblastoma Cells via PTEN/PI3K/AKT Signaling
    Article Snippet: .. Dissociated single cells were incubated with CD133-conjugated magnetic microbeads (CD133 MicroBead Kit, Miltenyi Biotec, Bergisch Gladbach, Germany), following which CD133+ cells were isolated per the manufacturer’s guidelines. ..

    Article Title: TREM1 is essential for maintaining stemness of liver cancer stem-like cells in hepatocellular carcinoma
    Article Snippet: .. CD133 + cells were isolated using the CD133 Microbead Kit (130-097-049, Miltenyi Biotec). .. Briefly, cells were incubated with CD133 microbeads and then passed through an LS column (130-042-401, Miltenyi Biotec) placed in the magnetic field of a MACS separator (130-042-302, Miltenyi Biotec) attached to a MACS multistand (130-042-303, Miltenyi Biotec).

    Article Title: hsa-miR-1246 is Consistently Overexpressed in Spheroid-Derived Cancer Stem Cells From Multiple Tumor Types
    Article Snippet: .. To confirm the presence of the CSC phenotype, we isolated the cells using the CD133 MicroBead Kit (human; Catalog #130-097-049 by Miltenyi Biotec), as CD133 is a marker of CSCs. .. Briefly, cells are labeled with CD133 MicroBeads, incubated, and then separated using a MACS column under a magnetic field.

    Article Title: Glioma-Associated Mesenchymal Stromal/Stem Cells Derived Exosomal miR-191 Promotes the Proneural-to-Mesenchymal Transition in Glioblastoma Cells via PTEN/PI3K/AKT Signaling
    Article Snippet: .. Dissociated single cells were incubated with CD133-conjugated magnetic microbeads (CD133 MicroBead Kit, Miltenyi Biotec, Bergisch Gladbach, Germany), following which CD133 + cells were isolated per the manufacturer’s guidelines. ..

    Incubation:

    Article Title: Glioma-Associated Mesenchymal Stromal/Stem Cells Derived Exosomal miR-191 Promotes the Proneural-to-Mesenchymal Transition in Glioblastoma Cells via PTEN/PI3K/AKT Signaling
    Article Snippet: .. Dissociated single cells were incubated with CD133-conjugated magnetic microbeads (CD133 MicroBead Kit, Miltenyi Biotec, Bergisch Gladbach, Germany), following which CD133+ cells were isolated per the manufacturer’s guidelines. ..

    Article Title: Glioma-Associated Mesenchymal Stromal/Stem Cells Derived Exosomal miR-191 Promotes the Proneural-to-Mesenchymal Transition in Glioblastoma Cells via PTEN/PI3K/AKT Signaling
    Article Snippet: .. Dissociated single cells were incubated with CD133-conjugated magnetic microbeads (CD133 MicroBead Kit, Miltenyi Biotec, Bergisch Gladbach, Germany), following which CD133 + cells were isolated per the manufacturer’s guidelines. ..

    Article Title: Mechanisms of HIV Latency in Hematopoietic Progenitors: GFI1 as a Key Regulator
    Article Snippet: Mononuclear cells were separated by Ficoll-Paque (Cytiva, cat# 17144002) density gradient centrifugation and either cryopreserved in 7.5% bovine serum albumin (BSA, Life Technologies, cat# 501215315) and 10% DMSO (Hybri-Max, Sigma-Aldrich, cat# D2650) in PBS or processed immediately. .. For HSPC enrichment, cord blood mononuclear cells were incubated in Stemspan SFEM II (StemCell Technologies, cat# 09655) for 2 hours to remove adherent cells, after which nonadherent cells were sorted for CD133+ cells using a CD133 MicroBead kit (Miltenyi Biotech, cat# 130100830) protocol. .. Additionally, anonymized presorted HSPCs were obtained from AllCells (cat# mLP RegH) as mobilized CD34+ cells (Donor 2).

    Purification:

    Article Title: An HTLV-1-Infected Humanized Mouse Model Expressing HLA-A*02:01 Demonstrates Effective CTL-Mediated Suppression of HTLV-1
    Article Snippet: Mononuclear cells (MNCs) were isolated from umbilical cord blood by density gradient centrifugation using Lymphoprep (Serumwerk Bernburg, Bernburg, Germany), following the manufacturer’s instructions. .. CD133 + cells were subsequently purified from the MNCs using a CD133 MicroBead Kit (Miltenyi Biotec, Tokyo, Japan), as per the manufacturer’s protocol. ..

    Magnetic Cell Separation:

    Article Title: Targeting UBE2T by β-elemene inhibits prostate cancer stem cells and bone metastasis by blocking the TRIM28/pGSK3β/β-catenin signaling.
    Article Snippet: .. To detect the tumorigenicity of PCSCs, the CD133+ PC3 cells were screened from the PC3 cells using magnetic bead sorting method (MACS) and CD133 MicroBead Kit (130–100–857, Miltenyi), and then the CD133+ PC3 cells were resuspended in matrigengel. ..

    Marker:

    Article Title: hsa-miR-1246 is Consistently Overexpressed in Spheroid-Derived Cancer Stem Cells From Multiple Tumor Types
    Article Snippet: .. To confirm the presence of the CSC phenotype, we isolated the cells using the CD133 MicroBead Kit (human; Catalog #130-097-049 by Miltenyi Biotec), as CD133 is a marker of CSCs. .. Briefly, cells are labeled with CD133 MicroBeads, incubated, and then separated using a MACS column under a magnetic field.



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    COP1 knockdown reduces motility, and stemness in HCC cells. (a‐b) Migration (a) or invasion (b) assay showing reduced motility in Huh7 and HepG2 cells treated with COP1‐siRNA, as quantified by the relative migration or invaded area. Statistical significance: *, p < 0.05; ** p < 0.01; *** p < 0.001 vs. control. (c) Sphere formation assay images and quantitative data from Huh7 and HepG2 cells, demonstrating a decrease in both the number and size of spheres in COP1‐siRNA‐treated cells. (d) Sphere formation assay images and quantitative data from PLC/PRF/5 <t>CD133+</t> cells. Compared to the control grpup, both the sphere number and size decreased upon COP1‐siRNA treatment. Scale bar, 100 μm Statistical significance: *, p < 0.05; *** p < 0.001 vs. control.
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    COP1 knockdown reduces motility, and stemness in HCC cells. (a‐b) Migration (a) or invasion (b) assay showing reduced motility in Huh7 and HepG2 cells treated with COP1‐siRNA, as quantified by the relative migration or invaded area. Statistical significance: *, p < 0.05; ** p < 0.01; *** p < 0.001 vs. control. (c) Sphere formation assay images and quantitative data from Huh7 and HepG2 cells, demonstrating a decrease in both the number and size of spheres in COP1‐siRNA‐treated cells. (d) Sphere formation assay images and quantitative data from PLC/PRF/5 <t>CD133+</t> cells. Compared to the control grpup, both the sphere number and size decreased upon COP1‐siRNA treatment. Scale bar, 100 μm Statistical significance: *, p < 0.05; *** p < 0.001 vs. control.
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    COP1 knockdown reduces motility, and stemness in HCC cells. (a‐b) Migration (a) or invasion (b) assay showing reduced motility in Huh7 and HepG2 cells treated with COP1‐siRNA, as quantified by the relative migration or invaded area. Statistical significance: *, p < 0.05; ** p < 0.01; *** p < 0.001 vs. control. (c) Sphere formation assay images and quantitative data from Huh7 and HepG2 cells, demonstrating a decrease in both the number and size of spheres in COP1‐siRNA‐treated cells. (d) Sphere formation assay images and quantitative data from PLC/PRF/5 <t>CD133+</t> cells. Compared to the control grpup, both the sphere number and size decreased upon COP1‐siRNA treatment. Scale bar, 100 μm Statistical significance: *, p < 0.05; *** p < 0.001 vs. control.
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    Image Search Results


    COP1 knockdown reduces motility, and stemness in HCC cells. (a‐b) Migration (a) or invasion (b) assay showing reduced motility in Huh7 and HepG2 cells treated with COP1‐siRNA, as quantified by the relative migration or invaded area. Statistical significance: *, p < 0.05; ** p < 0.01; *** p < 0.001 vs. control. (c) Sphere formation assay images and quantitative data from Huh7 and HepG2 cells, demonstrating a decrease in both the number and size of spheres in COP1‐siRNA‐treated cells. (d) Sphere formation assay images and quantitative data from PLC/PRF/5 CD133+ cells. Compared to the control grpup, both the sphere number and size decreased upon COP1‐siRNA treatment. Scale bar, 100 μm Statistical significance: *, p < 0.05; *** p < 0.001 vs. control.

    Journal: Genes to Cells

    Article Title: Functional Role of COP1 Gene in Hepatocellular Carcinoma Lipid Metabolism and Stemness

    doi: 10.1111/gtc.70108

    Figure Lengend Snippet: COP1 knockdown reduces motility, and stemness in HCC cells. (a‐b) Migration (a) or invasion (b) assay showing reduced motility in Huh7 and HepG2 cells treated with COP1‐siRNA, as quantified by the relative migration or invaded area. Statistical significance: *, p < 0.05; ** p < 0.01; *** p < 0.001 vs. control. (c) Sphere formation assay images and quantitative data from Huh7 and HepG2 cells, demonstrating a decrease in both the number and size of spheres in COP1‐siRNA‐treated cells. (d) Sphere formation assay images and quantitative data from PLC/PRF/5 CD133+ cells. Compared to the control grpup, both the sphere number and size decreased upon COP1‐siRNA treatment. Scale bar, 100 μm Statistical significance: *, p < 0.05; *** p < 0.001 vs. control.

    Article Snippet: The cells were treated with FcR Blocking Reagent and CD133 MicroBeads (130‐100‐857, Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer's instructions and incubated for 15 min at 4°C in the dark.

    Techniques: Knockdown, Migration, Control, Tube Formation Assay